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Hi! I need to create a growth curve for my sample of Vibrio splendidus. I've done the growth curve a few times and have reached log phase OD600 readings of anywhere between 0.71-1.03, when I'm typically expecting log phase of 0.8ish. I've repeated this experiment about six times and am wondering if the log phase can start to plateau at different OD600 depending on the culture. I've been growing mine samples up in TSB+2%NaCl, but have used differenet batches of it.
Has anyone else experienced this?
