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MegaEgo

Member Since 30 Jul 2012
Offline Last Active Aug 21 2012 06:34 AM
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Posts I've Made

In Topic: Oligos that do NOT bind in mouse genome

31 July 2012 - 11:56 PM

Yes, thanks, you helped me a lot :)

In Topic: Oligos that do NOT bind in mouse genome

31 July 2012 - 05:40 AM

The point is that my primer should not bind anywhere in genome. I'm not working with a specific mouse gene, but I will work with random fragments of mouse genome. So I'm not sure that if I do differentiation assay will help me much, I would have to input the whole mouse genome.

But definitively I will use high Tms, that's a good idea.

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