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You could use a vibratome, but you might be better off using a cryostat or regular sectioning using a microtome, especially as the tissues are already fixed in formaldehyde. Note that formaldehyde fixes often lower or remove native GFP fluorescence.
Either method will work well. However, the PCR will only detect the presence of DNA, unless you do some reverse transcription-PCR to detect transcript, the presence of DNA doesn't indicate that GFP would be expressed there. YOu could also do western blots on the tissues.
#146462 Vibratome (~ 150um thick slice) to identify GFP signal in the tissues
Posted
on 08 December 2012 - 12:21 PM
#140772 How to identify beta pancreatic cells in dish of heterogenous cells?
Posted
on 05 September 2012 - 05:18 PM
Great news
