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Dear all,
I am currently extracting RNA from circulating PBMCs.
My current experiment requires that I extract RNA at 10 minute intervals.
As it takes around 30 minutes for me to extract RNA (Qiagen RNeasy), it is clear that I really can't perform my experiment without some help.
I was wondering whether anyone had used buffer RLT to disrupt their cells, and then placed them on ice until a time when the rest of the RNA extraction protocol could be followed?
If not, then any other suggestions would be most welcome.
Many thanks in advance.
