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Not Telling
Greetings every one,
I am planning to do a Real-Time PCR, and I thought of using Pfaffl method to normalize my results, Since the amount of samples I can get from my subjects are very limited, I can not use them to prepare serial dilution and get the standard curve to calculate the efficiency.
I wonder if I can pool samples of some of my controls, and get the standard curve using that.
Cheers and thank you
