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Exactly. Incomplete repression from the associated repressor.
#154041 leaky promoter
Posted
on 21 April 2013 - 06:45 PM
#142670 solubility of adenine
Posted
on 02 October 2012 - 02:24 PM
Roughly speaking, the answer is that it is barely soluble. Adenine is soluble at 20 mg/ml in 1 M HCl, or the hydrochloride is similarly soluble.
#140555 Southern blotting
Posted
on 03 September 2012 - 12:47 PM
It doesn't affect the transfer usually. I have always stained my Southern blots with EtBr prior to transfer and never had a problem. It may slightly retard the blotting step, but with the times (overnight) usually used for this procedure, it shouldn't be a problem.
