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Female
Sorry if this is a repeat topic, I have looked/searched.
I have a vector that I am trying to give dual resistance to depending on cell line I want to express in.
A coworker said I could just put the puromycin cassette into the MCS but I am just wondering if this will cause problems? Two genes in one multiple cloning site?
It's a C1 vector but if I put a stop codon after the first gene, will the second one still express?
#153696 Two genes, same MCS
Posted
on 13 April 2013 - 12:44 PM
