That's actually the exact pH range I'd like to be in, so if I can just add it to the media then that's perfect. 7.5 is a little on the high end, but I think this will be the result of having a higher concentration of HEPES in the media. Most people recommend a range of 10-25 mM HEPES. So I'd guess that 10 mM will get me something around pH 7.2, while 25 mM might get me around 7.5 or so. Thanks for the help! Last question: If the pH is too HIGH, do I add HCl to take it down? Every site mentions NaOH or KOH, but no one ever mentions adding acids to bring the pH down. I'm a bit confused by this.
I'm actually planning to buy it as a 1M solution: http://www.invitroge...ents/hepes.html. I'm looking at the 100 mL volume and plan to put 10 mL of 1M stock into 1000 mL of media to get a final concentration of 10 mM HEPES. In that case, would I still need to add the NaOH? Or do I just dump the 10 mL into my media and continue making it as usual?
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