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mdarley

Member Since 12 Jul 2012
Offline Last Active May 08 2013 08:12 AM

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My research interests
Personal Care Chemistry
Cell Culture
Efficacy Testing

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bambum
13 Jul 2012 - 12:03

Topics I've Started

What size CO2 tank to purchase?

16 July 2012 - 04:26 AM

Hello,

I am setting up a new tissue culture lab and while I have tissue culture experience I do not have experience setting a lab up or major equipment purchasing. We have a Forma 3110 CO2 incubator and I need to get a CO2 tank.

Can anyone tell me what size tank to get?

Thank you,

Meghan

How to quantify/visualize cell surface proteins without FACS

13 July 2012 - 07:18 AM

Happy Friday!

But I have a bit of a conundrum...

I have recently graduated and taken a new job starting up a tissue culture/efficacy testing lab at a personal care company. I've been trained as an immunologist so my go to has been FACS but I do not have that equipment in my new lab.

I was to look at cell adhesion molecules but need a new way to do it. I have a BioTek microplate reader that can do all kinds of things. I'm thinking I could possibly prep my cells as if I was doing a FACS experiment, plate them in a 96 well plate, and read the fluorescence on the microplate reader.

Does anyone have any suggestions on the above idea or other types of assays I could do?

Thanks!

Meghan

ORAC assay - Hydrophobic Powder Dissolving Problems

12 July 2012 - 11:41 AM

Hey All,

I am running an ORAC (oxygen radical absorbance capacity) assay and I'm having some problems.

I have a hydrophobic powder that I want to test but it has to be in solution to do so... and it will not go into solution. I have tried Acetone, Methanol, and Chloroform with no luck. It falls out of solution almost immediately after mixing. I have attempted some heating and on a rocker plate for 1 hour. When the movement stops, it falls out and the heating didn't seem to change much.

Can anyone give me some tips on a better solvent to use or ORAC advice in general?

Thank you,

Meghan