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In order to make sure that you refold your protein correctly, what I would do is to steadily decrease the amount of urea you've got. So I'd go from 6 to 5,3,1 and 0M. What you could do is to use a spin concentrator in order to get rid of the excess buffer, and then resuspend it in the buffer with a lower amount of urea. But then I leave my protein for an hour between each stage, just to give it time.
It is slightly time consuming, but it tends o work well.
Best of Luck!
