alexa594

I'm hoping for some lore clarification.  I've tried a google search and have not had much luck.

I am interested in generating Cre/+;flx/+ mice as rapidly as possible and unfortunately I only have a single flx/flx mating pair to work with who have yet to produce any litters (our collaborator was short on mice and very kind to send us any).  I recall some lore from a previous lab that using Cre positive female mice in a mating scheme could be dangerous - I think this was due to the possibility of Cre losing gene specificity and being ubiquitously expressed.  I believe that this was a strain specific problem as I know people who use female Cre mice in their matings to get the genotypes they expect.  I have asked current lab members about this but have gotten varied answers: one person believes that using female Cre mice in a mating causes the Cre to be lost and another person says that using female Cre mice is simply a bad idea.  I would like to know what precisely the danger of using female Cre mice is and if it is in fact strain specific so that I can start testing the Cre strains I want to use to see if there is a problem using them.