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Hi,
After using Bradford method of protein determination, I diluted the samples with water to bring a final concentration of the samples to 1 μg/μl. However, after doing a gel electrophoresis for control with Beta-Actin, I came to know that my samples have different amount of proteins, probably due to an error in the bradford method.
I want to determine the protein concentration again in my diluted samples. Is that possible even after adding sample buffer? If yes, how?
If not, how do I concentrate the proteins in my sample and re-determine the protein concentration?
Thanks
