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Hey,
We've managed to work this out in the lab. Alkaline phosphatases lose biological activity when heated at 65 Celsius for 30 minutes, and oddly enough MDP maintains biological reactivity when treated under the same condition. Although this is MDP in Mili-Q, not sure how a complex matrix like NPA may effect MDP at these temperature, but I doubt there will be an effect.
So now to heat all my samples!!
