Find content
Not Telling
I want to do stainings on FFPE tissues using an antibody specific for a phosphorylated protein. Now as a control I want to show that this antibody really only binds to the phosphorylated protein. I've heard this can be checked by using a phophatase treatment, after which the staining should be negative. But I'm having a hard time finding a protocol, could anyone help me out?
Cheers,
Chris
