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biokot

Member Since 07 Jul 2012
Offline Last Active Jul 07 2012 12:58 AM
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Posts I've Made

In Topic: Relative promoter strength

07 July 2012 - 12:57 AM

Another article describes the relative strength of a number of promoters in several cell types.
http://www.plosone.o...al.pone.0010611
As you may know a list of factors determines the final out put, which is the amout of protein in the cell. Promoter sequence is only one of these factors.
Thus, it is highly recomemded to optimized the conditions for every single gene of interest. We know that most of the it's not possible in the lab, because of time, money and experience limitations.
But some companies do perform a screening of conditions. So it's recomended to look if any of the companies produce you protein. Some relevant details and even protocols sometimes are available.

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