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RNAses are incredibly stable, I don;t know if they will work in the CTAB buffer, but that is a common place for DNA extractions in general to have the RNase step, so I would give it a go there, adding after the 65 deg incubation has cooled to 37 or below.
#139508 Incorporating RNAse A into DNA extraction
Posted
on 15 August 2012 - 04:54 PM
#137421 CTAB of Fungi
Posted
on 11 July 2012 - 03:27 PM
That protocol should give you nice DNA. I suspect that you have added the final TE too soon after removing the ethanol - meaning that there is still some residual ethanol, which will prevent the DNA going back into solution. After you have removed the ethanol just leave the tubes (inverted on a tissue) on the bench to dry, if you can still smell the ethanol, then you should let them dry some more. Most protocols say to leave for 10 min, but I have found that 1-2 hours is usually fine.
Isopropanol pellets are usually clear or white (some protein carry over usually) and gel-like, and should be attached to the side of the tube near the bottom, instead of being directly in the bottom. If you place all the tubes in the microfuge with the hinge side to the top, the will always know where the pellet is/should be - directly down from the hinge, near the bottom of the tube.
Isopropanol pellets are usually clear or white (some protein carry over usually) and gel-like, and should be attached to the side of the tube near the bottom, instead of being directly in the bottom. If you place all the tubes in the microfuge with the hinge side to the top, the will always know where the pellet is/should be - directly down from the hinge, near the bottom of the tube.
