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csalat

Member Since 02 Jul 2012
Offline Last Active Jul 17 2012 05:16 AM
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Topics I've Started

Can I use MOPS buffer instead of PIPES in bacteria cells?

17 July 2012 - 05:19 AM

I am following the Current Protocols in Molecular Biology to make competent cells with the CaCl2 method. To prepare the CaCl2 solution they use PIPES as a pH buffer. Do someone know if I could use MOPS instead of PIPES for this application?

Thanks!!

How to distinguis soluble from insoluble recombinant proteins produced in E. col

02 July 2012 - 01:01 AM

Hii! I am gonna transform E.coli with a plasmid to express the protein and I need to know whether it is soluble or insoluble. I know that I should open the cells, separate soluble and insoluble fractions by centrifigation and load each on the SDS polyacrylamide gel. But I would truly appreciate if anyone could give me an effective protocol to do that.

Thanks!

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