Find content
Not Telling
hi guys.
FYI, i was preparing my freezing medium using FBS plus 10% of DMSO. I sterile-filtered my DMSO via PES membrane filter straight into my FBS and to my surprise, the PES membrane material was dissolved in DMSO... As so, i assume that the freezing medium i prepared earlier on contains not only FBS and DMSO, but also the PES membrane...
In this case, can i still use the freezing medium, after some kind of re-filtering, etc? Or should i just discard the whole bottle of freezing medium?
Thanks...
Topics I've Started
SNP genotyping of whole gene
04 July 2012 - 02:57 AM
Hi guys.
I am having a small problem here...
My project is to extract and analyse some SNP (single nucleotide polymorphism) of two genes, namely BDNF (brain-derived neurotrophic factor) and ApoE (apolipoprotein E) of human subjects. I am kinda lost on the feasible (yet not too expensive) ways to do so. For BDNF, the whole gene is 247-AA long & 26kDa, located at 11p13; while it is 317-AA & 36kDa, located at 19q13.2 for ApoE. My samples are human whole blood samples.
Is it possible if i would like to selectively cut and sequence only the 2 specific genes via PCR, and send the whole gene for sequencing (since BDNF is only app. 750bp, and ApoE is only app. 950bp long)? What types of PCR should i use?
Thanks
Regards,
Tony.
I am having a small problem here...
My project is to extract and analyse some SNP (single nucleotide polymorphism) of two genes, namely BDNF (brain-derived neurotrophic factor) and ApoE (apolipoprotein E) of human subjects. I am kinda lost on the feasible (yet not too expensive) ways to do so. For BDNF, the whole gene is 247-AA long & 26kDa, located at 11p13; while it is 317-AA & 36kDa, located at 19q13.2 for ApoE. My samples are human whole blood samples.
Is it possible if i would like to selectively cut and sequence only the 2 specific genes via PCR, and send the whole gene for sequencing (since BDNF is only app. 750bp, and ApoE is only app. 950bp long)? What types of PCR should i use?
Thanks
Regards,
Tony.
