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Hi all,
Very recently I am observing that fusion plates are forming clumps ( 2nd/3rd day) and hybridomas started dieng.... I tried changing all possible reagents and even new vial of Sp2/O has been thawed freshly for fusion though the result is same, cell started clumping, but the control wells are fine (where only spleen and only Sp2/O plated in HAT). Spleen is from immunized Balb/C mice... Can any one please suggest .....
