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rock&science

Member Since 28 Jun 2012
Offline Last Active Mar 21 2013 05:07 PM
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Topics I've Started

protein not stained by Coomassie Blue

15 October 2012 - 06:17 PM

Hi guys, just wondering if you all know whether it's possible that Coomassie Blue cannot stain certain protein.

does EGFP dimerize?

23 August 2012 - 04:46 PM

Hi all, does EGFP dimerize? I know that wild type GFP has a tendency to dimerize, is it the same with EGFP?

Thanks!!!

any tips for doing Fluorescence Western Blot?

12 August 2012 - 07:32 PM

Hi guys, our lab decided to change our Western blot approach to fluorescence western blotting. Any tips for this newbie? Thanks

what will happen if you let your affinity column beads dry?

07 August 2012 - 10:16 PM

does anyone know what will really happen? I know that it's going to wreck the column, but what does actually happen? thanks

mamallian expression vector that has FLAG tag and GFP

01 August 2012 - 04:56 PM

Hi,

Just wondering whether anyone aware of an expression vector that has C-terminal FLAG-tag and GFP at the same time? I have been googling with no luck. Thanks

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