thank u for pdf...i want protocol for extraction procedure for protein from green gram seed and its leaf....TCA extraction is fine or need any other method to b followed?
hi i think your appratus shows some leaking from sides when u add water...so seal it well with agar or agarose...and can also check gel concentration ....generally stacking gel is not as hard as resolving gel due to less concentration of acrylamide...but it sud polymerised as should not fall even if u envert the appratus...
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