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pcrman, on 31 July 2012 - 05:48 PM, said:
There is no doubt that your PCR system is contaminated. You said you replaced many reagents, but have you also ordered new primers yet?
Also be aware that contamination may not only come from reagents, but also from your work bench, pipettes etc. You should clean up those things and assemble your PCR in a designed area just for PCR. Using aerosol resistant tips is also important to get rid of contamination.
Also be aware that contamination may not only come from reagents, but also from your work bench, pipettes etc. You should clean up those things and assemble your PCR in a designed area just for PCR. Using aerosol resistant tips is also important to get rid of contamination.
I made fresh aliquots from original stocks so I guess my stock could be contaminated, but then why no band in my untransfected cell? I keep an area designated for PCR and wipe it down, wipe pippettes down, use special tips etc. And why does it run exactly where my other bands do?
