Find content
Not Telling
The problem I´m dealing with is a contamination in my S. cerevisiae cultures (GG 595, W303, BY4741) of unknown nature which suddenly occurred in our lab some months ago. It looks like G+ motile cocci swimming in the culture. All of our attempts to isolate them (by straining or in semi-liquid media) have been unsuccessful. They´re also resistant to ampicilin, kanamycin and chloramphenicol. Yeast cells in the presence of contaminants shrivel up and don´t grow well (especially, when used for transformation - LiAc method).
Do you have any suggestions? Which species this could be and how to identify them?
Thanks a lot.
