.anka

Hi,

I have been trying to isolate RNA FFPE tissue with Qiagen-FFPE RNeasy Kit. After isolation I checked samples 1% and 1,5% native agarose gel and I saw just one band. I expect to see 2 band, 28S RNA and 18S RNA, but there was just one.

I used 6X DNA loading dye instead of RNA loading dye, so it may be due to this problem?

Nano drop readings, A260 ratio is 461ng/ul and A260/A280 ratio is 1,95. So I don't know whether it is good or bad because generally people says approximately 30ng/ul and I doubt DNA contamionation because of high concentration. Why I have seen just one band? I repeat isolation with different samples but all times I saw same band and same size? What should I to see both of 28S and 18S RNA?

I attach my gel photo which run today. Help me, what do you think?

(first well is 100bp DNA ladder, second well is sample)

Thanks