Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

LacquerHead

Member Since 21 Jun 2012
Offline Last Active Apr 24 2013 12:50 PM

Find content

Community Stats

Group Active Members
Active Posts 21
Profile Views 422
Member Title member
Age Age Unknown
Birthday Birthday Unknown
Gender
Not Telling

About me

My research interests
Neuroscience

Contact Information

2 Neutral

Friends

LacquerHead hasn't added any friends yet.

Latest Visitors

2xzwei
15 Apr 2013 - 04:21

Topics I've Started

SAP dephosphorylation

18 April 2013 - 04:30 AM

Hi all,

Wondering at what stage SAP is best used, following gel purification of the vector cut by a single enzyme, I have done it right after digestion with BamHI and have been having trouble getting a ligated product with my insert, guessing that Im getting recircularization of the vector?
Thanks.

Determining in frame in modified vector

12 April 2013 - 06:02 AM

Hi, if I add novel restriction sites to a vector backbone to accept an insert, what is the best way to assess if my vector modification has kept everything in frame? Thanks.

Deleting restriction enzyme sites

12 April 2013 - 04:46 AM

Hi,

What is the currently best accepted method to do this in a plasmid? Need to delete a site in the middle of my insert that is crucial for ligating that insert into the vector. Thanks!

Creating primers to add restriction sites to vector backbone

11 April 2013 - 05:26 PM

Hi all,

I need to add restriction sites to where a single BamHI site sits on my backbone to be able to ligate an insert into that spot, no MCS on this plasmid. I have not done this and am wondering if anyone has experience modifying the recipient vector with additional sites? Any rules/strategies for primer design to amplify my 8kb vector by PCR and add two unique sites? Thanks.

Mouse genotyping issues

13 March 2013 - 02:59 AM

Hi all,

Wondering if you can help. I am having some issues with genotyping, using a traditional KO LacZ gene targeting strain - and although I get exactly the predicted bands for LacZ, WT and both for het that the original paper predicted I am at times still seeing protein and transcripts of the knocked out protein in the tissue of a KO mouse. I am wondering if years of inbreeding may have introduced some mutation, not really sure how to test this. Any ideas would really help, thanks!

Google Sign in options