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#139938 How OD600 influence on IPTG induction and protein expression
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on 24 August 2012 - 10:07 PM
Bacteria follows the Lag, Log, stationary and decline phase of growth in a limited amount of medium. If you have to take bacteria in log phase of growth so it express good. More overnight culture you take means you are running risk of taking O.D. for Stationary / Decline phase culture (overnight O.D.) than from log phase.
