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El Crazy Xabi

Member Since 12 Jun 2012
Offline Last Active May 23 2013 11:01 PM

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My research interests
Microbiology, microbial ecology, extremophiles, astrobiology

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Topics I've Started

viability/integrity stain of fungal cells

12 November 2012 - 03:50 PM

Hi guys,

Do you know about stainings that could be used to evaluate the cell integrity or viability of fungal cells in bright field or fluorescence microscope?

Thanks!!

5 M NaCl stock

29 October 2012 - 02:04 PM

Hi guys,

I was trying to make a 5 M NaCl stock but I have problems to dissolve it.

I added 292.2 g to about 750 mL MQ initially but after 2 hours didn't dissolve so I added more water and heat it up. I even reach to boiling to dissolve but did't work either. Even worse, the solution surface started to cristalize ¬¬'...
It's the first time I have to prepare this stock and I'm a little bit paranoid already Posted Image

, maybe the balance I used does not work properly or the bottle had another chemical Posted Image

?

I don't know, having a problem with this and not with other really complex solutions pfff

If anybody has any suggestion trick or whatever, it would be good

Chloramphenicol storage life

25 September 2012 - 10:47 PM

Hi guys,

I have some fungal isolates I'm working with and I'm trying to get rid of potential satellite bacteria growing with them using chloramphenicol (Cam).

I haven't had any problems with most of them but one of the cultures seems to be contaminated with some slimy central-spore forming bacteria. I used originally a stock in ethanol 1 month old so I repeated it with fresh solution with the same result.

The Cam was already in the lab and it has been there in the fridge for years (though in the bottle says to store it at RT), but I cannot find info about storage life of the pure solid Cam. Do you know something about it?

Thanks.

Cellophane on agar plate for fungi

31 July 2012 - 08:51 PM

Hi guys,

Reading an article from Fomina et al. (2007)* I found a technique I could find to be quite old but I didn't know. The technique consists in placing a piece/disk of cellophane directly on the agar surface which allows the fungi to access to the nutrients but avoids the hyphae to penetrate the agar, allowing to remove all the fungal biomass whenever you want. It is quite useful for biomass harvesting, biochemical profiling of single species, selecting during isolation on base to certain metabolic activity,...

Most of the new references don't give a proper reference of this cellophane sheets and the old ones refer to discontinued products. Many of these products can have hydrophobic coatings or other treatments that may turn them unusable for this technique. So, I was wondering if anybody has ever used this technique and could give me some orientation.

Thanks!

*Fomina, M., Charnock, J. M., Hillier, S., Alvarez, R. & Gadd, G. M. (2007). Fungal transformations of uranium oxides. Environ Microbiol 9, 1696–1710.

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