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Tween80 as lipid source? Do you add the lipid mixture too?
What bacteria do you use? usually they aren't that lipophilic, except a few that produce a hydrophobic coating, and these are susceptible to the presence of surfactants like Tween80
Tween80 is used as surfactant in media containing hydrophobic substances (e.g. hydrophobic C-sources like PAHs). Did you test the media with only Tween80 to say they can use it?
#154495 Test for production of antimicrobial substances by bacteria
Posted
on 01 May 2013 - 07:13 PM
#145715 Identification of new species
Posted
on 22 November 2012 - 04:05 PM
Why not? I have a fungal isolate from Australia which has 100% identity with another from Japan and other from China, and based on the ITS which is much more variable.
Did you isolate the second time from dog too? Same medium?
Some species have a plethora of closely related strains or within the same species, almost like a gradient of similarity based on 16S. Others however, may have the exact same 16 S or maybe just one bp different and being different species. The last case is not very common but there are some cases that need extra sequencing of some other genes to confirm.
Did you isolate the second time from dog too? Same medium?
Some species have a plethora of closely related strains or within the same species, almost like a gradient of similarity based on 16S. Others however, may have the exact same 16 S or maybe just one bp different and being different species. The last case is not very common but there are some cases that need extra sequencing of some other genes to confirm.
#140072 Drug recrystallize when i added to DMEM
Posted
on 27 August 2012 - 05:34 PM
I used to work with PAHs (phenanthrene, pyrene,...) and we disolved the compound in acetone. However if you added to a medium without a surfactant (I used Tween80), it appeared as cloudy due to thin precipitation but concentration was 100 uM in the media, 1000 times higher than yours. It also depends on your compound, medium,...
If you give details of compound, culture media,... would be easier to give a proper response
If you give details of compound, culture media,... would be easier to give a proper response
#138674 Creating a growth curve for bacteria- reaching various log phases
Posted
on 31 July 2012 - 07:08 PM
Do you use the same exact size of inoculum? number of cells, UFC,...
Does the inoculum come from the same origin/culture medium with the same age, temperature, agitation speed, volume,...?
Do you get the inoculum from the log phase?
The growth curve is dependent on all those parameters
Does the inoculum come from the same origin/culture medium with the same age, temperature, agitation speed, volume,...?
Do you get the inoculum from the log phase?
The growth curve is dependent on all those parameters
#138310 Change in the pH of stock solution
Posted
on 26 July 2012 - 12:10 AM
Ruth Grace, on 25 July 2012 - 12:32 AM, said:
Thank you for your opinion, pal. I did not autoclave the stock. Is it necessary to autoclave them?
Don't know if it is necessary, but some solutions can change the pH before and after autoclaving
One msds of IAA (link) states that is light sensitive.
You say you autoclave the seawater before adding the IAA, but the IAA... is it sterile? Some bugs may hide in the IAA flask
#138242 Change in the pH of stock solution
Posted
on 24 July 2012 - 09:59 PM
Some poorly buffered media may show a different pH when are stirred due to the CO2. Try to measure the pH both direct from the bottle and using the magnetic stirrer. If you notice a change, it's because of the air.
Did you autoclave after preparation? pH can change.
Also, some solutions, buffers and media may take a day to stabilize the pH, e.g. adjusting the pH of the Tris-HCl 1M requires initial pH adjustment and repeat it after 24h to get the desired stable pH value
Did you autoclave after preparation? pH can change.
Also, some solutions, buffers and media may take a day to stabilize the pH, e.g. adjusting the pH of the Tris-HCl 1M requires initial pH adjustment and repeat it after 24h to get the desired stable pH value
