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Coating antigen overnight at 4 centigrade diluted with autoclaved PBS only.
Block the plates with 3% BSA in 0.1% PBST for 1 hour room temperature.
Primary A.b = Culture Supernatant
Sec A.b = Anti Human IgG Alkaline phosphatase labelled
Detection Reagent = Phosphate substrate solution. 1 hour room temperature.
