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DaviQd

Member Since 11 Jun 2012
Offline Last Active Sep 20 2012 08:26 PM

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About Me

David Halvorsen completed his BSc in Biochemistry at SUNY Plattsburgh in 2011. After completing an internship at the SENS Foundation he was hired as a full time staff member. David is working, alongside the other OncoSENS team members, to decipher the alternative lengthening of telomeres mechanism. He is mentoring two of the summer intern students on assessing age-related microglial functional changes at the RC and is guiding students on the Microglia Differentiation AI Review.

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Mountain View, CA

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David Halvorsen
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OncoSENS
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SENS Foundation
My research interests
Oncology

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Website URL http://davidhalvorsen.brandyourself.com/

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DNA Index?

11 June 2012 - 06:01 PM

Greetings,

I'm attempting to determine relative telomere length (RTL) using Dako's Telomere PNA Kit <http://www.dako.com/...d_products.htm> and am having trouble understanding what the term DNA index means. The formula for RTL on Page 9 of the protocol <http://www.dako.com/...ctid=114791002> requires the DNA index of my control & sample cells. The ratio of aneuploid/euploid appears to be what "DNA index" refers to. Is this correct?

Earlier in the protocol I'm told to gate for G0/1 cells [using a FL-A vs FL-W plot]. If I've already gated for all the 2C cells, why does the formula require DNA index? This seems redundant to me, but I'm probably just misunderstanding something.

Thanks everyone!

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