Could you please let me know what is the EDTA concentration needed for the detachment of the HaCaT cells. I was trying 0.5 mM, and it was without succes.
A second attempt was with 0.25% Trypsin-EDTA solution, 5 minutes at 37C and only few cells were detached.
Thanking you in advance,
ldoreliasimonaMember Since 08 Jun 2012
Offline Last Active Jun 17 2012 10:38 AM
- Group Members
- Active Posts 1
- Profile Views 190
- Member Title member
- Age Age Unknown
- Birthday December 17
ldoreliasimona hasn't added any friends yet.
17 Dec 2012 - 01:00