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Female
Hi all,
Could you please let me know what is the EDTA concentration needed for the detachment of the HaCaT cells. I was trying 0.5 mM, and it was without succes.
A second attempt was with 0.25% Trypsin-EDTA solution, 5 minutes at 37C and only few cells were detached.
Thanking you in advance,
Simona
