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  2. → Viewing Profile: Posts: janapix

janapix

Member Since 08 Jun 2012
Offline Last Active Feb 11 2013 04:57 AM
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Posts I've Made

In Topic: vaccine take

09 February 2013 - 12:09 AM

I'm going to receive this very vaccination quite soon!
My lab supervisor was talking about a couple of lab members to simply didn't 'take' the vaccine when it was administered. He mentioned
that one of them had been immunized just because she had worked with VACV for so long... so a nasty scab area didn't appear on her
skin (or on the skin of the other guy... who didn't "take" the vaccine either)

In Topic: how so T-cells recognise peptides in ICS assays?

03 February 2013 - 12:31 AM

Am I right to think that macrophages and dendritic cells from the spleen itself simply take up the peptides and
channel them to MHC-I on the surface through cross-presentation?

I'd be very grateful for some information on the molecular biology behind the ICS assay, when I'm testing individual peptides.

Thanks.

In Topic: Why doesn't the transcriptome reflect the proteome?

09 June 2012 - 03:48 PM

Excellent. mRNA held in pools? That's something new - I'd love a brief explanation if you, or anyone else has the time.
I've also added:
-transcriptome includes rRNA, tRNA and non-coding RNAs which will  not give functional protein.

I wish there were more 'technical' reasons for the discrepancy though. I think it would be rather poor to include poor technique as an answer.

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