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  2. → Viewing Profile: Posts: DaisyCarbine

DaisyCarbine

Member Since 05 Jun 2012
Offline Last Active Jun 13 2012 08:35 AM
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Posts I've Made

In Topic: Bizarre changes in plasmid band size

06 June 2012 - 06:34 AM

It's the same prep used in every gel. Also, I digested it with restriction enzymes -- shouldn't linearizing the plasmid get rid of supercoiling?

In Topic: Blunt-cloning -all are vector self-ligation products?

05 June 2012 - 09:45 AM

I've had good luck using antarctic phosphatase in blunt-ended ligation reactions -- you can heat-inactivate it at 65 C, so you don't have to purify the DNA before setting up your ligation rxn.

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