Natasuki26

Hi everyone, pls help me with the anaylisis of my western blot bands. The case is at follows, i must compare the expression of a certain protein X in control mice, sick mice, and mice resistant to the desease. For each type of mice i have 5 protein samples. With each sample (15 in total) i have made western blot so now i have the bands for my X protein for each sample, and also their respective loading control, actin. With these bands i put the image in the program image J and obtain the intensity of each band. Now i dont know how to proceed. In examples of how to analyze western blot bands they typically use 1 control and 1 experimental band, with wich they normalize the experimental bands to the control and the actin band, but i have 5 samples for control, so i dont know how to analyse my data, should i just take a mean of each group of samples? I need to do statistics later to see if it is significant the differential expression. Pls help me with the analysis!!
thks =)