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Arun Kumaran

Member Since 01 Jun 2012
Offline Last Active Jul 11 2012 07:35 AM

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My research interests
Proteomics, Genomics

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proteaMatt
04 Jun 2012 - 05:59
researchgal
01 Jun 2012 - 09:40

Posts I've Made

In Topic: Antibody recognition principle

01 June 2012 - 10:42 AM

1.Monoclonal antibodies are good for specific recognition.
2.Align your epitope region with other proteins or BLAST for similarity. Sometimes, some antibodies can recognize most related epitopes. You can use that antibody for your proteins.

In Topic: Protein extraction

01 June 2012 - 10:35 AM

sigma protease inhibitor cocktail is more efficient than other protease inhibitor cocktails.

In Topic: i have problem with TCA/aceton percipitation

01 June 2012 - 10:30 AM

CHAPS is zwitterionic detergent. SDS is an anionic surfactant. CHAPS cannot be replaced by SDS sometimes. It may affect the pH which cause poor soluble of albumin (Albumin is moderately soluble in alkaline condition).

In Topic: What is the yeild expected for an efficient IP?

01 June 2012 - 10:17 AM

I got ~0.3% only from plant tissue. It doesn't matter for me since it is enough for further process. Try to use more beads.

In Topic: Immunoprecipitation with two antibodies

01 June 2012 - 10:08 AM

You can use 0.1 M NaCl (pH 11.3) to separate protein complex from antibody. After eluted, you have to neutralize it.