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yourGrandma

Member Since 30 May 2012
Offline Last Active Jul 16 2012 06:47 AM
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Topics I've Started

need help with gst-tagged protein-purification

06 June 2012 - 04:57 AM

hey guys!

i have a problem.

i expressed a protein in insect cells and now try to purify it. it is gst tagged and has a molecular weight of 120kDa.
everything works just fine but i am not able to elute the protein. if i check via westernblot it is still located on the glutathion-sepharose-beads.
i tried with an elution-buffer containing 40mM Glutathion.
do you have an advice for me?

thanks a lot!

problem with purification of gst-tagged protein

06 June 2012 - 04:54 AM

hey guys!

i have a problem.

i expressed a protein in insect cells and now try to purify it. it is gst tagged and has a molecular weight of 120kDa.
everything works just fine but i am not able to elute the protein. if i check via westernblot it is still located on the glutathion-sepharose-beads.
i tried with an elution-buffer containing 40mM Glutathion.
do you have an advice for me?

thanks a lot!

i need help with virus pcr

30 May 2012 - 03:53 AM

i'm new here and i have an problem:
i created baculogold viruses for infecting FS21 cells with a transfection kit from bd with recombinant dna. now i want to know if it is possible to make pcrs with this viruses. do the viruses crack during 95°C??

thanks for helping

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