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  2. → Viewing Profile: Posts: Indhumathi

Indhumathi

Member Since 28 May 2012
Offline Last Active May 13 2013 08:19 PM
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Posts I've Made

In Topic: will mRNA from different cell line lead to loss of expected product in RT PCR?

17 April 2013 - 11:19 PM

Hi
I have checked the isoforms, the sizes are same for all isoforms. may be i should check the splicing. but alternative splicing wont differ within species right?
Indhu

In Topic: PCR with Plasmid recovered from filter paper

01 April 2013 - 10:39 PM

thanks again for the reply.

In Topic: PCR with Plasmid recovered from filter paper

31 March 2013 - 09:27 PM

hi
Thanks for your reply. plasmid is not in FTA card, its just in filter paper.

In Topic: PCR with Plasmid recovered from filter paper

27 March 2013 - 09:02 PM

hi leelee,
Thanks for the reply. I am planning to use part of it for transformation and part of it for PCR to confirm the presence of the required portion in the plasmid before going for transformation. The sender did not mention the amount of plasmid present in the circle. I read somewhere it usually would be around 2 ug. How much of sterile water I should use to extract the plasmid?

In Topic: PCR - consistent false positive results

11 October 2012 - 11:29 PM

hi al,
sorry for the late response. I found out the real problem was with our primers. I have got a new set of primers synthesized and have finished the first set of cloning. thanks for your suggestions.
Indhu

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