When I put out the DNA I put it into -20 for one hour and then centrifuged for 30 min and washed with 70% ethanol and redissolved in H2O (because of the next using in cells) but the concentration was small again, about 40 ng/ul ...so I think when it was in -80 it didn´t work, DNA is not damaged but the precipitation doesn´t work in these conditions.
Find content
Female
