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Tracy0110

Member Since 22 May 2012
Offline Last Active Jul 22 2012 09:24 PM

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Molecular cloning

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Nayeem991
23 Jul 2012 - 03:49

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In Topic: Digest for insert orientation

22 July 2012 - 07:29 PM

I think I've worked this one out for myself. By digesting with either Xho or Sal and digesting with an enzyme which cuts within my insert, I can work out which way it has gone in depending on the sizes I get from the digestion