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@rkay447 (or others:-))
How do you expose a film overnight? Doesn't something (I don't know where - perhaps the blot itself) go bad? If I am seeing a clear signal in my input but a weak signal in my IP lane, could this mean that I should expose overnight? I would think not, since my protein is very clear in the input (it'd get overexposed I presume).
I have to add to this topic by saying that I have problems with protein precipitation during my clearing of the lysate before putting it onto the beads and I ALWAYS use fresh lysate, never freeze it at any point.
Any thoughts on this?
