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In Topic: An improve method for making Competent Cells without heat shock. How?
19 May 2012 - 10:19 AM
Hi!
The biggest problem for me is the heat shock. Once, I tried to use eppendorfs tubes, but didn't work. So, I chose PCR microtubes because of the "thin wall" (to get a homogeneous heat faster) . But I'll try your protocol with 2 ml eppendorfs! You have any tip about this for me?
About the electroporation, we don't have an electroporator in our lab
Surely, is the best way!
Thanks for the help!
The biggest problem for me is the heat shock. Once, I tried to use eppendorfs tubes, but didn't work. So, I chose PCR microtubes because of the "thin wall" (to get a homogeneous heat faster) . But I'll try your protocol with 2 ml eppendorfs! You have any tip about this for me?
About the electroporation, we don't have an electroporator in our lab
Surely, is the best way! Thanks for the help!
