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Koeng

Member Since 16 May 2012
Offline Last Active Dec 17 2012 03:43 PM

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Viruses

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bioforum
30 Nov 2012 - 14:33
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Posts I've Made

In Topic: FREE DNA Sequencing

09 July 2012 - 06:09 PM

Hello,

In Topic: A good vector to make gene library?

06 July 2012 - 04:40 PM

Also you can use them because of the "blue white" screen. The MCS is inside of the lacZ gene, so plasmids that where sucessfully ligated will be white, while the un-ligated ones will still be blue.

-Koeng

In Topic: A good vector to make gene library?

05 July 2012 - 04:16 PM

I am new too, but I would use either pUC18 or pUC19, because they give high yields of plasmids. Good luck with that!

-Koeng

In Topic: Hypothetical Synthetic Plasmid

04 July 2012 - 12:21 PM

Thank you Pito! I do have all summer and this is enjoyable, and I am going to see if I can create a plasmid with a better purpose then just being small. I will post when I complete it. Thank you!!!

-Koeng

In Topic: Hypothetical Synthetic Plasmid

03 July 2012 - 03:20 PM

Yes I know what lacz is important for. (Lactose metabolism) I mean when I say "I just want ampicillin in the agar plates" because if I had the lacz gene, I would have to put X-gal in the solution to make it turn blue. And yes I meant bla promoter. I would just like some input, like "you should do this or that", to continue this fun project. (well, fun for me). Maybe some nice MCS sites from different plasmids, if you remember that plasmid just say it and post I can look it up. Or maybe a different resistance, because that resistance gene is better or shorted or because personally when you where in high school the ampiciilin in your plates didn't work but Kanamycin worked really well.

Also thanks for the idea of some new programs, I am looking into them and will probably spend the rest of the night making a really nice map. But right now I'll just paste the pdf of the map I make with ApE.

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