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I have not worked with the cell line you are working with, but with THP1 monocytes I have previously got differentiation to macrophages within 3 days of incubation with 1uM PMA. Hope this is helpfull. Sorry if it does not answer your question.
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In Topic: How to induce early apoptosis in cells without using chemicals or UV
08 October 2012 - 04:51 PM
madelingirly, on 02 September 2012 - 10:34 PM, said:
Could u check this method
A good positive control for apoptosis: Heat shock the cells. Incubate 1 minute @ 56°C followed by incubation @ 37°C
dependent on cell type. Usually 1 hour @ 37°C after shock is sufficient to induce 50% apoptosis for most cell lines
A good positive control for apoptosis: Heat shock the cells. Incubate 1 minute @ 56°C followed by incubation @ 37°C
dependent on cell type. Usually 1 hour @ 37°C after shock is sufficient to induce 50% apoptosis for most cell lines
Thank you Very much for proposing this method. I tried it on K562 and HL60 cells as well as PC3M and I get apoptosis. I tried both 56°C and 60°C and 37°C incubation thereafter and 56°C gave me 50% apoptosis out of which about 40-45 % was in late apoptosis and remarkably 60°C gave me 90% apoptosis (late apoptosis). This is an amazing method. Thank you for proposing it. Thanks once again.
