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im going to amplify a dna fragment, and i am planning to clone it into a particular plasmid/vector, and i can only do so by using only one ristriction site/enzyme as all the rest cut through my insert (pcr -product). my question is, how would i go about designing primers for my insert so i can use only one ristrection site?
any ideas would be much appreciated ! thanks alot
oh extra information - im using Gibson assembly to stick all my pcr products together.
