Find content
Not Telling
Hi madina,
The idea for using both renilla and firefly in the dual luciferase is so you can normalise your different conditions to the control luciferase.
I therefore say, you could trust your normalised results.
I assume you did 3 separate transfection experiments, not splitting your cells in 3 and simply use the same sample 3 times?
Each experiment is slightly different, with different cell numbers, pipetting mistakes, transfection efficiencies and incubation/growing times. You therefore do not necessarily get the same result for the control luciferase each time you repeat the experiment, to control for this you are supposed to normalise the data to your renilla.
#133201 Luciferase assay-normalisation
Posted
on 20 April 2012 - 07:49 AM
