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extra chunk? I assume you ran your purified protein on a gel and you are getting multiple bands. The best way to get the protein that you want from this mixture is size exclusion by FPLC. See if you have a column available and try that.
#133901 How to get pure protein of a gene
Posted
on 02 May 2012 - 08:01 AM
#133603 How to get pure protein of a gene
Posted
on 26 April 2012 - 01:24 PM
PCR out the gene using primers with appropriate restriction sites, then clone the PCR product into your vector of choice.
