agorganic

Hi fella's,

Thanks.

I ended up setting the thermocycler to finish @ 4 degrees C (forever). I set the electrophoresis for 70 volts over 30 minutes, the amps were set at 400, although the BIO-RAD powerpac seems to alter the amps depending on the volts entered when ran.

I was rightly disappointed when looking under the UV, it looks like there is a problem shown here, where the ladder is on the left and then the 5th well is the PCR'd gDNA sample with no restriction enzyme which was used as a control against the same sample which was restriction enzyme digested in thermocycler. Therefore all the other wells are not displaying anything....

The agarose gel I made had ethidium bromide in it. Except for the ladder, all samples placed into the well had the x6 loading dye, even the control.

My research supervisor suggested digesting with more DNA. I'll add some more useful information associated with this once I've had more of a chance to go through the finer details, as I'm now working in a casual position with a grain storage company over the southern hemisphere harvest.

Also I need to add the 6x loading dye. The gel has got the ethidium bromide in it.

Thanks guys. I left the information fairly simple in regards to the genetic database, as far as I know the University don't have anything formal for livestock genetic database, I know they record alot of detail about individual animals which they show at the Royal Mebourne Showgrounds. Generally the best and worst animals are measured for beef meat quality and carcass conformation, though this involves slaughtering the animals, therefore my research could be useful towards this aspect.

Last year I was privy to the process of cataloguing the beef information, which tends to be based on a range of industry standard values and formulas, together with photographs of different cuts from various animals. The lecturer who handled this has since gone into retirement and I believe the system is now being managed exclusively by another department, I'll need to investigate this further with them and what level of involvement the agriculture diploma students have.

Undoubtedly my research project supervisor has developed a database for his department (viticulture) and I will query him further about that.

Hi Curtis,

Sorry I was a bit tired when I posted this and had edited my question before posting. Do you feel like critiquing the data in the last 2 sheets? Is there any other calculations I should be aware of for PCR?

Not particularly confident of the next steps, so difficult to know what data can be compiled from a PCR?

Can I write it as 100:2000, as this format is what I'm referring to specifically.

Thank you.