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PVB

Member Since 13 Apr 2012
Offline Last Active Jan 13 2013 01:19 AM
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Topics I've Started

difficulty in solubilizing L-cycloserine in water

30 December 2012 - 05:19 AM

Hi all !!!

I am facing difficulty in dissolving L-cycloserine in water. Manufacturer recommends to dissolve the antibiotic in water, and so does several research articles. But, I am not able to do so. Some literatures mention about dissolving the antibiotic in 1:9 ethanol. This method also did not solve my problem. Kindly help.


Thanks in advance

PVB

bacteriostatic assay... troubleshooting!!!

03 November 2012 - 02:14 AM

Hi all!!!


A monoclonal antibody generated against outer membrane proteins of my microbe has bacteriostatic activity. To evaluate this activity statistically, I am standardizing a bacteriostatic assay in microtitre plate (basically, recording the absorbance on hourly basis for 8 continuous hours). At early stage of standardization, things worked out very well. But I'm facing a wierd problem now. Positive control (organism in enrichment broth) which used to give a typical bacterial growth curve, is showing a drastic decrease in absorbance @ the first hour of incubation itself, and no change in absorbance in further hours. Tried everything to get rid of the problem - used fresh broth, tried different enrichment broths, fresh culture... no improvement. Not finding out the root of the problem. Kindly help.


Regards,
PVB

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