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Hi all, I'm planning to use alizarin red staining assay to observe and quantify bone formation on my scaffolds.. Will it be the same procedure as being done on a plate/well. For quantification purpose, we use the de-staining solution and take results using a plate reader. Is it necessary to scrape cells? I cannot scrape cells from a scaffold.
Thank you
P.S. : I'm new to tissue eningeering..
