hogthehedge

Oops, just saw how blurry the image shows. Hyperlink here: https://dl.dropbox.c...7/lehninger.jpg

Thanks ascacioc, I talked to him today and said I would like to be given due credit if he used any of those images. It took some assertiveness on my part, but he finally agreed that if anything is used in a paper, or in presentations, or on the lab website, there will be an acknowledgement to my contributions. We agreed that I would leave behind one copy of all data and take one copy with me. I haven't signed anything, I don't think any of the other students have either. Maybe it's only the PIs who have to sign something like that?

I'm sorry to seem so negative about this guy, but I haven't had a great experience working for him. (This is the venting and counselling forum, yes?)
In general, he is so dismissive of student work, and has very little positive to say about anything shown to him. It's not even like he's particularly demanding or a perfectionist, or that he does pathbreaking work. He's unable to appreciate the good work that others do in the lab. All his grad students seem to dislike him too! (They didn't even want him in the group photo. Geez.)

Thanks Akdor and ascacioc, I think you're right in that talking to him directly will be the best. I don't think he will listen, but at least I will have tried.
Could anyone tell me the general policy on this? If someone takes images in a lab, whose property are they? The lab's, or the image-taker's?

Hi MM,

Do you use serum? If yes, do you filter it? Sometimes there's a lot of debris in serum. I thought I had contamination once (also primary neuronal cultures), but it was just a lot of gunk that came with unfiltered serum.

HogtheHedge

Hi Bob, thanks for your reply.

It was under 200 ug/ul maintenance selection during expansion. I have upped it to 500. Can you please explain a little bit about the effects of methylation?
Are the non-fluorescent cells only expressing the resistance gene and not my gene of interest? How shall I handle this?